Pharmaceutical sciences
Warsito Warsito; Elvina Dhiaul Iftitah; Nashi Widodo; Nuryamin Kazal; Lucy - Arianie
Abstract
Several thiourea derivative compounds have been used as inhibitors of Plasmodium falciparum via the plasmepsin inhibition pathway. The thiourea derivative (compound 3) namely 1-{1-(3,4-dimethoxyphenyl)-4.6-bis[(3,4-dimethoxyphenyl)methyl]heptane-2-yl]-3-{[ (2E)-3-phenylprop-2-en-1-ylium-1-yl]amino}thiourea ...
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Several thiourea derivative compounds have been used as inhibitors of Plasmodium falciparum via the plasmepsin inhibition pathway. The thiourea derivative (compound 3) namely 1-{1-(3,4-dimethoxyphenyl)-4.6-bis[(3,4-dimethoxyphenyl)methyl]heptane-2-yl]-3-{[ (2E)-3-phenylprop-2-en-1-ylium-1-yl]amino}thiourea was synthesized using the one-pot synthesis method by comparing the renewable starting reagents Methyl Isoeugenol (MIE) and Methyl Eugenol (ME). Observation using FTIR and LCMS-MS was shown that ME is more representative as a precursor. Compound (3) was synthesized using ME isothiocyanate compound (2), hydrazine, and cinnamaldehyde at 70 °C for 5 h, yielding 0.2769 g (22.47 %), and the molecular ion is 740 (98 % area). In vitro bioactivity tests against Plasmodium falciparum 3D7 of compound (3) resulted in IC50=10.19 ppm. Molecular docking of ligand (3) gave an insight that the compound (3) was a promising anti-Plasmodium falciparum compound.
Biological sciences
Afrah Talib Abdullah; kadhim F Kadhim
Abstract
The objective of this study was to isolate and genetically identify keratinophilic fungi associated with humans, test their susceptibility to nystatin, and evaluate the effectiveness of Methanol Extract (ME) from chamomile flowers against these fungi. No cytotoxic effects were observed in the extract ...
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The objective of this study was to isolate and genetically identify keratinophilic fungi associated with humans, test their susceptibility to nystatin, and evaluate the effectiveness of Methanol Extract (ME) from chamomile flowers against these fungi. No cytotoxic effects were observed in the extract cytotoxicity assay using human blood solution. The antifungal activity of the chamomile extract varied among different fungal isolates and extract concentrations. The extract exhibited a strong inhibitory effect on fungal growth, depending on the type of fungal strain and concentration. Statistical analysis revealed significant differences between the effects of the extract and the conventional antifungal medication (nystatin) on specific fungi. GC/MS analysis of the chamomile extract identified several bioactive compounds, including flavonoids, phenolic compounds, and essential oils. The presence of these bioactive components in the extract likely contributes to its high antifungal activity against the tested fungal infections.
Biological sciences
noah mahmood; zaynab abdulghany
Abstract
Coronavirus disease 2019 (COVID-19) represented a highly respiratory syndrome to worldwide. Aim of current research was to determine the changes in hematology and inflammatory parameters into three categories: non-infected with COVID-19 as control groups, infected with COVID-19 and cured from the disease ...
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Coronavirus disease 2019 (COVID-19) represented a highly respiratory syndrome to worldwide. Aim of current research was to determine the changes in hematology and inflammatory parameters into three categories: non-infected with COVID-19 as control groups, infected with COVID-19 and cured from the disease in Baghdad center. Current study was proved that the levels of hematology, inflammatory and liver enzymes were upregulated in infected with COVID-19 group and decreased to normal levels in cured groups. The Receiver Operating Characteristic curve (ROC) curve analysis presented that d-dimer marker and AST were the most accurate markers. The AUROC of d-dimer and AST were 0.747 and 0.760, respectively. And the OR 95 % CI of d-dimer and AST were (0.677-0.817) and (0.694-0.827), respectively. These findings give a highlight on d-dimer and AST as an important biomarkers in monitoring COVID-19 detection.
Biological sciences
Saghar Bakhtiari; Fatemeh Noorbakhsh; Sadaf Mohseni Fard; Raheleh Safaei javan
Abstract
The aim of this research was to investigate the effect of Zataria multiflora and silver nanoparticles on the biofilm formation ability of A. baumannii and the expression of genes involved in this process. Thirty isolates of A. baumannii bacteria were collected and studied from the Milad Hospital of Tehran. ...
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The aim of this research was to investigate the effect of Zataria multiflora and silver nanoparticles on the biofilm formation ability of A. baumannii and the expression of genes involved in this process. Thirty isolates of A. baumannii bacteria were collected and studied from the Milad Hospital of Tehran. The effects of Zataria multiflora essential oil and silver nanoparticles on the biofilm formation process were analyzed using the microtiter plate method. Also, the gene expressions of pil and aba after exposure to nanoparticles and essential oil were measured. Treatment the strains with Zataria multiflora essential oil at the MIC concentration, along with silver nanoparticles, reduced biofilm formation by the strains assessed and the expression of the genes noted. It seems that Z.multiflora essential oil and silver nanoparticles, can be used as candidate drugs in treatment or disinfection.
Biological sciences
Manal Hadi Ghaffoori Kanaan; Hiba Riyadh Al-abodi; Sura Saad Abdullah; Mojtaba Memariani; Maryam Kohansal; Abdolmajid Ghasemian
Abstract
In this study, fifty Moraxella species were isolated. The antibiotic susceptibility, β-lactamases and minimum inhibitory and bactericidal concentrations were evaluated. The rate of ESBLs among M. catarrhalis, M. osloensis and M. lacunata included 47 % (n=11), 36.84 % (n=7) and 37.5 % (n=3), respectively. ...
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In this study, fifty Moraxella species were isolated. The antibiotic susceptibility, β-lactamases and minimum inhibitory and bactericidal concentrations were evaluated. The rate of ESBLs among M. catarrhalis, M. osloensis and M. lacunata included 47 % (n=11), 36.84 % (n=7) and 37.5 % (n=3), respectively. The strong biofilm formation was observed among 52 %, 63.5 % and 62.5 % of M. catarrhalis, M. osloensis and M. lacunata, respectively. The rate of the bro1, bro2 and blaCTX-M1 genes in M. catarrhalis included 23/23 (100 %), 12/23 (52.17 %) and 5/23 (21.8 %), respectively. Moreover, 14/19 (73.68 %), 8/19 (42.10 %) and 2/19 (10.52 %) of M. osloensis, respectively contained these genes. The bro1 gene was detected in all the M. lacunata isolates. Moraxella spp highly produced BRO β-lactamases and ESBLs, and deciphered strong biofilm formation.
Pharmaceutical sciences
Noor Adnan Najm; Ekhlas Sabah Hassan
Abstract
Sepsis is now more commonly regarded as an uncontrolled inflammatory and immune response throughout the body, triggered by microbial invasion and resulting in high mortality rate from organ damage. This study aimed to demonstrate the protective impact of ipragliflozin on lung damage induced in mice by ...
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Sepsis is now more commonly regarded as an uncontrolled inflammatory and immune response throughout the body, triggered by microbial invasion and resulting in high mortality rate from organ damage. This study aimed to demonstrate the protective impact of ipragliflozin on lung damage induced in mice by Cecal Ligation and Puncture (CLP). The levels of lung tissue inflammatory & oxidative stress markers (IL-1β, IL-6, TNF-α, NF-KB & MDA) were significantly elevated in the sepsis in comparison to the sham. On other hand, mice treated with ipragliflozin had a significant reduction in the level of these mediators. Histological examination revealed significant lung tissue damage in mice undergo sepsis which significantly reduced after ipragliflozin treatment. Ipragliflozin shows promise in reducing pulmonary dysfunction in male mice with sepsis.